Bürkert - Close to you
Office /2
    • Main Office
      Burkert Middle East FZE
      Ras al Khaimah | United Arab Emirates

      Phone +971 7207 6696
      Fax +971 7207 6695
      E-Mail fnyrf.nr@ohexreg.pbz
    • Distributor
      Daly Fluid Technologies LLC – DFT
      Dubai | United Arab Emirates
      Phone +971 4887 8185
      Fax +971 4887 8184
      E-Mail fnyrf@qsgzr.pbz
  • Main Office
  • Headquarters
  • Systemhaus
  • Distributor
  • Office
  • Plant
  • Logistic center
bürkert
We make ideas flow.
We make ideas flow.

Liquid chromatography

"Chromatography" is an umbrella term covering physical methods in which substances are separated via distribution between a stationary and mobile phase.

HPLC (High-Performance Liquid Chromatography) is a separating process in which a liquid sample is transported through a stationary phase under high pressure. Depending on the nature of the interaction between the phases and the sample, we distinguish between adsorption chromatography, partition chromatography, ion-exchange chromatography, sizeexclusion chromatography and affinity chromatography. The processes of adsorption chromatography and partition chromatography are the chief processes used in HPLC. HPLC uses partition particles with grain sizes of a few µm; it thus achieves high numbers of plates or trays, but at the same time, requires a high counter-pressure to be overcome during transport of the mobile phase through the thin separation column (of a few millimeters diameter). All parts must be interconnected without any dead volume wherever possible and must be pressure-stable (up to 300 bar).

My Comparison List